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Purposecontains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genes
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 110809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDG
- Backbone size w/o insert (bp) 21845
- Total vector size (bp) 21851
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV3B cap genes, AAV2 rep genes, adenovirus helper genes
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Alt nameAAV3B nonstructural protein Rep78, capsid protein VP1
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Speciesadenovirus, adeno-associated virus
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Insert Size (bp)3086
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GenBank IDAF028705.1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (not destroyed)
- 3′ cloning site Cla1 (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDGM3B was a gift from David Russell (Addgene plasmid # 110809 ; http://n2t.net/addgene:110809 ; RRID:Addgene_110809) -
For your References section:
AAV-mediated gene targeting methods for human cells. Khan IF, Hirata RK, Russell DW. Nat Protoc. 2011 Apr;6(4):482-501. doi: 10.1038/nprot.2011.301. Epub 2011 Mar 24. 10.1038/nprot.2011.301 PubMed 21455185
Map uploaded by the depositor.
