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pDGM3B
(Plasmid #110809)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110809 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDG
  • Backbone size w/o insert (bp) 21845
  • Total vector size (bp) 21851
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV3B cap genes, AAV2 rep genes, adenovirus helper genes
  • Alt name
    AAV3B nonstructural protein Rep78, capsid protein VP1
  • Species
    adenovirus, adeno-associated virus
  • Insert Size (bp)
    3086
  • GenBank ID
    AF028705.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (not destroyed)
  • 3′ cloning site Cla1 (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGM3B was a gift from David Russell (Addgene plasmid # 110809 ; http://n2t.net/addgene:110809 ; RRID:Addgene_110809)
  • For your References section:

    AAV-mediated gene targeting methods for human cells. Khan IF, Hirata RK, Russell DW. Nat Protoc. 2011 Apr;6(4):482-501. doi: 10.1038/nprot.2011.301. Epub 2011 Mar 24. 10.1038/nprot.2011.301 PubMed 21455185