164,349 results

1. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2260

   Plasmid

   #141998

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPomyces-SaCas9

   Plasmid

   #129553

   Purpose

   Contains codon-optimized SaCas9 and gRNA for streptomyces

   Depositor

   Mingzi M. Zhang, Fong Tian Wong

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   SaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PR_GEF YFP (2XPDZ-YFP-LARG DH)

   Plasmid

   #80408

   Purpose

   Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

   Depositor

   Michael Glotzer

   Article

   Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

   Insert

   2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRK248

   Plasmid

   #173755

   Purpose

   co-injection plasmid

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

   Plasmid

   #180267

   Purpose

   Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   EGFR sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBPHLWL-LexA

   Plasmid

   #26258

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   LexA
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

   Plasmid

   #171438

   Purpose

   Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-c-Jun
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE GFP-Grin2b KI

   Plasmid

   #131487

   Purpose

   Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Grin2b Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-CDT2

    Plasmid

    #215091

    Purpose

    Expresses FLAG-CDT2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDT2 (DTL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPM_Pveg-sfgfp

    Plasmid

    #121503

    Purpose

    Integration vector that expresses gfp in S. mutans

    Depositor

    Robert Shields

    Article

    Shields et al Appl Environ Microbiol. 2019 May 17. pii

    Insert

    Superfolder GFP
    

    Use

    S. mutans integration vector, integrates at the m…

    Tags

    Expression

    Mutation

    Promoter

    Pveg

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rluc8-NbV5

    Plasmid

    #201479

    Purpose

    BRET detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-Rluc8
    

    Use

    Tags

    Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAW62.YY1.FKBP.knock-in.mCherry

    Plasmid

    #104370

    Purpose

    For knocking in FKBP F36V P2A mCherry onto YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-mCherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHCMV_RVFV_GP

    Plasmid

    #207304

    Purpose

    Mammalian expression of Rift valley fever virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

    Plasmid

    #190077

    Purpose

    express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    ACE2 (ACE2, TMPRSS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. RED

    Plasmid

    #139835

    Purpose

    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

    Depositor

    Karim Mekhail

    Article

    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

    Insert

    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 protein contains two mutations: D10A and H8…

    Promoter

    tet-inducible CMV

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKDR-EF1a

    Plasmid

    #183567

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Unattenuated
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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