pLV-neo-CD2-FAK
(Plasmid #37013)

• Depositing Lab: Bob Weinberg
• Publication: Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10290-5. Epub 2009 Jun 5.

Backbone

• Vector backbone: pLV-IRES-neo
• Backbone manufacturer: I. Ben-Porath
• Modifications to backbone: pLV-IRES-neo (a gift from I. Ben-Porath) was generated by replacing dsRed2 in pLV-tTRKRAB-Red (Addgene plasmid 12250) with the neomycin-resistance gene and removing the sequence of tTR-KRAB.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CD2-FAK
• Alt name: CD2
• Alt name: FAK
• Species: H. sapiens (human)
• Entrez Gene: CD2 (a.k.a. LFA-2, SRBC, T11)
• Entrez Gene: PTK2 (a.k.a. FADK, FADK 1, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK)
• Promoter: EF1a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EF1a-fwd
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CD2-FAK was acquired from Dr. Steven Frisch (Frisch SM et al, 1996, JCB 134, 793).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry