168,579 results

1. C321.∆A ompT- rne- endA- lon-

   Bacterial Strain

   #182775

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Ampicillin

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

2. TurboID-V5_pRS415

   Plasmid

   #107167

   Purpose

   expresses V5-tagged TurboID in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Yeast

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Orai1 YFP

   Plasmid

   #19756

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Oct. 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-puro-CMV-CC3AI

   Plasmid

   #78909

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   CC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pREDCas9

   Plasmid

   #71541

   Purpose

   Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   lambda red genes
   plasmid curing system
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP-CL1

   Plasmid

   #11950

   Depositor

   Nico Dantuma

   Article

   Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

   Insert

   YFP-CL1
   

   Expression

   Mammalian

   Available Since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107307

   Purpose

   Encoding an SPH activation system

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Expression

   Mammalian

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-Sec16A

   Plasmid

   #36155

   Purpose

   Expresses Sec16A as a GFP fusion

   Depositor

   David Stephens

   Article

   Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

   Insert

   Sec16A (SEC16A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry2-N1

   Plasmid

   #54517

   Purpose

   Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   mCherry2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

    Plasmid

    #69909

    Purpose

    Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNestin-EGFP

    Plasmid

    #38777

    Depositor

    Wei Cui

    Article

    Noisa et al Stem Cell Rev. 2010 Sep;6(3):438-49.

    Insert

    nestin 2nd intron enhancer with TK mini promoter (NES Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF0630

    Plasmid

    #142668

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1I2 (NR1I2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-HRD1

    Plasmid

    #231688

    Purpose

    Expresses human HRD1 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    HRD1 (SYVN1 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-FR-GECO1a

    Plasmid

    #163681

    Purpose

    Far-red fluorescent calcium indicator FR-GECO1a

    Depositor

    Robert Campbell

    Article

    Dalangin et al bioRxiv 2020.11.12.380089

    Insert

    FR-GECO1a
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-hPAX7 (#348)

    Plasmid

    #78337

    Purpose

    Overexpression of human PAX7 ORF with a N-terminal FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    PAX7 (PAX7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-3z/601

    Plasmid

    #26656

    Depositor

    Jonathan Widom

    Article

    Lowary et al J Mol Biol. 1998 Feb 13. 276(1):19-42.

    Insert

    
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-TBK1-C-FLAG-HA

    Plasmid

    #131792

    Purpose

    Lentiviral vector expressing WT TBK1 containing STOP codon

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    STOP codon

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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