164,349 results

1. pcDNA3-myc-CUL3

   Plasmid

   #19893

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1479

   Plasmid

   #143504

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TP63 (TP63 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4-AMBRA1-3xFLAG

   Plasmid

   #174157

   Purpose

   Vector for expression of 3xFLAG-tagged wild-type AMBRA1

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1-GfaABC1D-lck-jGCaMP8s (AAV9)

   Viral Prep

   #176761-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8s (#176761). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8s plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   GfaABC1D

   Tags

   None

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE

   Plasmid

   #130991

   Purpose

   AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet-Kv2.1

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. M13-kmR

   Plasmid

   #206861

   Purpose

   M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)

   Depositor

   Irene Chen

   Article

   Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371

   Insert

   Kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX4T3-GST-Tev

   Plasmid

   #177142

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   Tev
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human ubiquitination-related proteins CRISPR KO library

   Pooled Library

   #174592

   Purpose

   CRISPR Cas9 KO library targets 660 ubiquitination-related proteins, including all major E1, E2, E3 and deubiquitinating enzymes, as well as other ubiquitination-related proteins.

   Depositor

   Juan Bonifacino

   Article

   Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

9. PAX6 donor plasmid

   Plasmid

   #105239

   Purpose

   Knock in H2B-GFP into the human PAX6 locus

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

   Insert

   PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX208 CMV sTurboID (C)-HA-FRB-ERM

    Plasmid

    #153007

    Purpose

    Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)-ERM (Cb5)
    

    Use

    Lentiviral

    Tags

    FRB and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105545

    Purpose

    AAV expression of EGFP-Cre from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HI

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK1009 (pSL9)

    Plasmid

    #191004

    Purpose

    Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 35S-GWY-VP16/pAM

    Plasmid

    #201232

    Purpose

    Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GWY-VP16
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. JD171

    Plasmid

    #229483

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cre-IRES-PuroR

    Plasmid

    #30205

    Purpose

    Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

    Depositor

    Darrell Kotton

    Article

    Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

    Insert

    Cre recombinase (cre Enterobacteria phage P1)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Flpo

    Plasmid

    #60662

    Purpose

    Expresses pCAG-Flpo; codon-optimized for mammalian expression

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Flpo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0208

    Plasmid

    #142556

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXP2 (FOXP2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-ABEmax-NRCH

    Plasmid

    #136923

    Purpose

    Expresses ABEmax-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    ABEmax-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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