CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3.
Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 2016 Feb 22. PubMed Article
Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 2016 Feb 22. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
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| 78804 | pcDNA3-HA-RIPK3 | To overexpress RIPK3 in Mammalian Cells |
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| 78805 | pcDNA3-HA-mRipk3 | To overexpress mRipk3 in Mammalian Cells |
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| 78806 | pcDNA3-HA-RIPK3 S199A | To overexpress RIPK3 S199A in Mammalian Cells |
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| 78807 | pcDNA3-HA-RIPK3 K55R | To overexpress RIPK3 K55R in Mammalian Cells |
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| 78808 | pcDNA3-HA-RIPK3 K89R | To overexpress -RIPK3 K89R in Mammalian Cells |
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| 78809 | pcDNA3-HA-RIPK3 K363R | To overexpress RIPK3 K363R in Mammalian Cell |
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| 78810 | pcDNA3-HA-RIPK3 K501R | To overexpress RIPK3 K501R in Mammalian Cells |
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| 78811 | pcDNA3-HA-RIPK3 2KR(55,363) | To overexpress RIPK3 2KR(55,363) in Mammalian Cells |
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| 78812 | pcDNA3-HA-RIPK3 2KR(89,501) | To overexpress RIPK3 2KR(89,501) in Mammalian Cells |
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| 78813 | pcDNA3-HA-RIPK3 4KR | To overexpress RIPK3 4KR in Mammalian Cell |
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| 78814 | pcDNA3-HA-RIPK3 V460P | To overexpress RIPK3 V460P in Mammalian Cells |
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| 78815 | pcDNA3-FLAG-RIPK3 | To overexpress RIPK3 in Mammalian Cells |
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| 78817 | pcDNA3-FLAG-RIPK3 K50A | To overexpress RIPK3 K50A in Mammalian Cells |
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| 78818 | pcDNA3-FLAG-RIPK3 S199A | To overexpress RIPK3 S199A in Mammalian Cells |
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| 78819 | pcDNA3-FLAG-RIPK3 2KR(55,363) | To overexpress RIPK3 2KR(55,363) in Mammalian Cells |
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| 78820 | pcDNA3-FLAG-RIPK3 2KR(89,501) | To overexpress RIPK3 2KR(89,501) in Mammalian Cells |
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| 78821 | pcDNA3-FLAG-RIPK3 4KR | To overexpress RIPK3 4KR in Mammalian Cells |
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| 78822 | pEGFP-N2-RIPK3 | To detect localization of RIPK3 protein in live mammalian cells |
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| 78823 | pEGFP-N2-RIPK3 2KR(55,363) | To detect localization of RIPK3 2KR(55,363) protein in live mammalian cells |
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| 78824 | pEGFP-N2-RIPK3 2KR(89,501) | To detect localization of RIPK3 2KR(89,501) protein in live mammalian cells |
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| 78825 | pEGFP-N2-RIPK3 4KR | To detect localization of RIPK3 4KR protein in live mammalian cells |
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| 78826 | pEGFP-N2-RIPK3 V460P | To detect localization of RIPK3 V460P protein in live mammalian cells |
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| 78827 | pGEX-4T-1-RIPK3 | To purify GST-tagged recombinant RIPK3 protein |
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| 78828 | pGEX-4T-1-RIPK3 4KR | To purify GST-tagged recombinant RIPK3 4KR protein |
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| 78829 | pBabe-puro-RIPK3 | retroviral expression of RIPK3 |
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| 78830 | pBabe-puro-mRipk3 | retroviral expression of mRIPK3 |
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| 78831 | pBabe-puro-RIPK3 2KR(55,363) | retroviral expression of RIPK3 2KR(55,363) |
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| 78832 | pBabe-puro-RIPK3 2KR(89,501) | retroviral expression of RIPK3 2KR(89,501) |
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| 78833 | pBabe-puro-RIPK3 4KR | retroviral expression of RIPK3 4KR |
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| 78834 | pcDNA3-HA-RIPK1 | To overexpress RIPK1 in Mammalian Cell |
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| 78835 | pcDNA3-HA-RIPK1 K115R | To overexpress RIPK1 K115R in Mammalian Cells |
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| 78836 | pcDNA3-HA-RIPK1 K163R | To overexpress RIPK1 K163R in Mammalian Cells |
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| 78837 | pcDNA3-HA-RIPK1 K316R | To overexpress RIPK1 K316R in Mammalian Cells |
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| 78838 | pcDNA3-HA-RIPK1 K571R | To overexpress RIPK1 K571R in Mammalian Cells |
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| 78839 | pcDNA3-HA-RIPK1 K604R | To overexpress RIPK1 K604R in Mammalian Cells |
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| 78840 | pcDNA3-HA-RIPK1 K627R | To overexpress RIPK1 K627R in Mammalian Cells |
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| 78841 | pcDNA3-HA-RIPK1 3KR | To overexpress RIPK1 3KR in Mammalian Cells |
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| 78842 | pcDNA3-FLAG-RIPK1 | To overexpress RIPK1 in Mammalian Cells |
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| 78843 | pcDNA3-FLAG-RIPK1 K115R | To overexpress RIPK1 K115R in Mammalian Cells |
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| 78844 | pcDNA3-FLAG-RIPK1 K163R | To overexpress RIPK1 K163R in Mammalian Cells |
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| 78845 | pcDNA3-FLAG-RIPK1 K316R | To overexpress RIPK1 K316R in Mammalian Cells |
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| 78846 | pEGFP-N2-RIPK1 | To detect localization of RIPK1 protein in live mammalian cells |
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| 78847 | pEGFP-N2-RIPK1 3KR | To detect localization of RIPK1 3KR protein in live mammalian cells |
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| 78848 | pEGFP-N2-RIPK1 I539D | To detect localization of RIPK1 I539D protein in live mammalian cells |
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