PurposeTo overexpress RIPK1 in Mammalian Cell
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78834||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneRIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA-RIPK1 was a gift from Jaewhan Song (Addgene plasmid # 78834 ; http://n2t.net/addgene:78834 ; RRID:Addgene_78834)
For your References section:CHIP controls necroptosis through ubiquitylation- and lysosome-dependent degradation of RIPK3. Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J. Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 2016 Feb 22. 10.1038/ncb3314 PubMed 26900751