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MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
(Plasmid #63729)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63729 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    no backbone--uses MiP intron for replication in bacteria
  • Vector type
    Mammalian Expression
  • Selectable markers
    RNA-OUT

Growth in Bacteria

  • Bacterial Resistance(s)
    RNA-OUT ( 6% sucrose)
  • Growth Temperature
    30°C
  • Growth Strain(s)
    RNA-OUT selection competent cells, such as NTC4862 DH5α competent cells (Nature Technology)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Lin28
  • Species
    H. sapiens (human)
  • Mutation
    codon optimized
  • Entrez Gene
    LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
  • Promoter SFFV
  • Tag / Fusion Protein
    • T2A (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Nanog
  • Species
    H. sapiens (human)
  • Mutation
    codon optimized
  • Entrez Gene
    NANOG

Cloning Information for Gene/Insert 2

Resource Information

Depositor Comments

In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIP 339 CoMiP coLin28 2A Nanog IRES eGFP was a gift from Mark Kay & Joseph Wu (Addgene plasmid # 63729 ; http://n2t.net/addgene:63729 ; RRID:Addgene_63729)
  • For your References section:

    Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC. Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081. 10.1038/srep08081 PubMed 25628230