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MIP 227 CoMiP 4in1 wo shRNA p53
(Plasmid #63727)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63727 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    codon-optimized minicircle (CoMiC) construct
  • Backbone size w/o insert (bp) 7608
  • Modifications to backbone
    codon-optimized expression cassette of the four canonical reprogramming factors OCT4, KLF4, SOX2, and c-MYC (OKSM)
  • Vector type
    Mammalian Expression
  • Selectable markers
    RNA-OUT

Growth in Bacteria

  • Bacterial Resistance(s)
    RNA-OUT ( 6% sucrose)
  • Growth Temperature
    30°C
  • Growth Strain(s)
    RNA-OUT selection competent cells, such as NTC4862 DH5α competent cells (Nature Technology)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mini-intronic-plasmid intron
  • Alt name
    pUC-RNA-OUT
  • Alt name
    MIP intron
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1355
  • Mutation
    codon-optimised
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter SFFV
  • Tag / Fusion Protein
    • IRES-dtomato (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIP 227 CoMiP 4in1 wo shRNA p53 was a gift from Mark Kay & Joseph Wu (Addgene plasmid # 63727 ; http://n2t.net/addgene:63727 ; RRID:Addgene_63727)
  • For your References section:

    Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Diecke S, Lu J, Lee J, Termglinchan V, Kooreman NG, Burridge PW, Ebert AD, Churko JM, Sharma A, Kay MA, Wu JC. Sci Rep. 2015 Jan 28;5:8081. doi: 10.1038/srep08081. 10.1038/srep08081 PubMed 25628230