Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. PubMed Article
Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62277 | pJZC518 | Yeast dCas9 expression plasmid |
| 62278 | pJZC519 | Yeast dCas9-VP64 expression plasmid |
| 62279 | pJZC522 | MCP-VP64 fusion |
| 62280 | pJZC504 | PCP-VP64 fusion |
| 62281 | pJZC506 | COM-VP64 fusion |
| 62282 | pJZC620 | Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells |
| 62283 | pJZC638 | Expresses MCP-VP64 and dCas9 in Yeast cells |
| 62313 | pJZC545 | sgRNA with 1x MS2 for yeast cells |
| 62314 | pJZC583 | sgRNA with 2x MS2 for yeast cells |
| 62315 | pJZC588 | sgRNA with 2x MS2 (wt+f6) for yeast cells |
| 62316 | pJZC548 | sgRNA with 1x PP7 for yeast cells |
| 62317 | pJZC603 | sgRNA with 2x PP7 for yeast cells |
| 62318 | pJZC572 | sgRNA with 1x com for yeast cells |
| 62319 | pJZC593 | sgRNA with MS2-PP7 for yeast cells |
| 62320 | pJZC523 | sgRNA for yeast cells |
| 62321 | pJZC625 | sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells |
| 62322 | pJZC595 | Expresses MCP-VP64 and dCas9 in Yeast cells |
| 62325 | pJZC35 | sgRNA for mammalian cells with mCherry marker |
| 62327 | pJZC32 | sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells |
| 62328 | pJZC25 | sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells |
| 62330 | pJZC33 | sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells |
| 62331 | pJZC34 | sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells |
| 62332 | pJZC41 | sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells |
| 62333 | pJZC39 | sgRNA + 1x PP7 with mCherry for mammalian cells |
| 62334 | pJZC40 | sgRNA + 2x PP7 with mCherry for mammalian cells |
| 62335 | pJZC101 | sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells |
| 62336 | pJZC48 | sgRNA + 1x COM with COM-VP64 effector for mammalian cells |
| 62337 | pJZC102 | sgRNA for mammalian cells with mCherry marker |
| 62338 | pJZC77 | sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells |
| 62339 | pJZC78 | sgRNA + 1x COM with COM-KRAB effector for mammalian cells |
| 62340 | pJZC103 | sgRNA for mammalian cells with mCherry marker |
| 62341 | pJZC73 | sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells |
| 62342 | pJZC74 | sgRNA + 1x COM with COM-KRAB effector for mammalian cells |
| 62344 | pJZC116 | sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP |
| 66564 | pJZC42 | sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry |
| 66565 | pJZC43 | sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry |