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Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds.
Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
62277pJZC518Yeast dCas9 expression plasmid
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62278pJZC519Yeast dCas9-VP64 expression plasmid
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62279pJZC522MCP-VP64 fusion
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62280pJZC504PCP-VP64 fusion
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62281pJZC506COM-VP64 fusion
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62282pJZC620Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells
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62283pJZC638Expresses MCP-VP64 and dCas9 in Yeast cells
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62313pJZC545sgRNA with 1x MS2 for yeast cells
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62314pJZC583sgRNA with 2x MS2 for yeast cells
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62315pJZC588sgRNA with 2x MS2 (wt+f6) for yeast cells
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62316pJZC548sgRNA with 1x PP7 for yeast cells
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62317pJZC603sgRNA with 2x PP7 for yeast cells
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62318pJZC572sgRNA with 1x com for yeast cells
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62319pJZC593sgRNA with MS2-PP7 for yeast cells
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62320pJZC523sgRNA for yeast cells
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62321pJZC625sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells
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62322pJZC595Expresses MCP-VP64 and dCas9 in Yeast cells
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62325pJZC35sgRNA for mammalian cells with mCherry marker
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62327pJZC32sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells
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62328pJZC25sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells
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62330pJZC33sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells
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62331pJZC34sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells
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62332pJZC41sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells
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62333pJZC39sgRNA + 1x PP7 with mCherry for mammalian cells
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62334pJZC40sgRNA + 2x PP7 with mCherry for mammalian cells
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62335pJZC101sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells
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62336pJZC48sgRNA + 1x COM with COM-VP64 effector for mammalian cells
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62337pJZC102sgRNA for mammalian cells with mCherry marker
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62338pJZC77sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
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62339pJZC78sgRNA + 1x COM with COM-KRAB effector for mammalian cells
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62340pJZC103sgRNA for mammalian cells with mCherry marker
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62341pJZC73sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
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62342pJZC74sgRNA + 1x COM with COM-KRAB effector for mammalian cells
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62344pJZC116sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
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66564pJZC42sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry
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66565pJZC43sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry
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