Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells.
Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di Ventura B
Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404. PubMed Article
Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di Ventura B
Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61342 | pDB22 | Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES) |
| 61343 | pDN34 | mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES) |
| 61344 | pDN41 | mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES) |
| 61345 | pDN42 | mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES) |
| 61346 | pDN43 | mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES) |
| 61347 | pDN77 | mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES) |
| 61349 | pDN80 | LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment. |