Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain.
Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. PubMed Article
Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106432 | pUBQ10::ZF108_3xFLAG_TET1cd | pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis |
| 106433 | pUBQ10::ZF2CACTA1_3xFLAG_TET1cd | pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis |
| 106434 | pUBQ10::ZF1CACTA1_3xFLAG_TET1cd | pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsis |
| 106435 | SunTag-FWAgRNA-4-22aa-TET1cd | SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter |
| 106436 | SunTag-FWAgRNA-4-14aa-TET1cd | SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter |
| 106437 | SunTagCACTA1g2-22aa-TET1cd | SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter |
| 106438 | SunTagng22aa | Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome. |
| 106439 | SunTagng14aa | Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome. |
| 106441 | pUBQ10::ZF108_3xFLAG_YPet | pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis |