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Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout.
Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120426EF1a_mCherry_P2A_Hygro_BarcodeBarcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120427EF1a_ASCL1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120428EF1a_ASCL3_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120429EF1a_ASCL4_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ASCL4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120430EF1a_ASCL5_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120432EF1a_CDX2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120433EF1a_CRX_P2A_Hygro_BarcodeBarcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120434EF1a_ERG_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120435EF1a_ESRRG_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120436EF1a_ETV2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120437EF1a_FLI1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120438EF1a_FOXA1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120439EF1a_FOXA2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120440EF1a_FOXA3_P2A_Hygro_BarcodeBarcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120441EF1a_FOXP1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express FOXP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120442EF1a_GATA1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120444EF1a_GATA4_P2A_Hygro_BarcodeBarcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120445EF1a_GATA6_P2A_Hygro_BarcodeBarcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120447EF1a_HAND2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120448EF1a_HNF1A_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120450EF1a_HNF4A_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120451EF1a_HOXA1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120452EF1a_HOXA10_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120453EF1a_HOXA11_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120454EF1a_HOXB6_P2A_Hygro_BarcodeBarcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120455EF1a_KLF4_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120456EF1a_LHX3_P2A_Hygro_BarcodeBarcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120457EF1a_LMX1A_P2A_Hygro_BarcodeBarcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120458EF1a_MEF2C_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120459EF1a_MESP1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120460EF1a_MITF_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120461EF1a_MYC_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120462EF1a_MYCL_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120463EF1a_MYCN_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120464EF1a_MYOD1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120465EF1a_MYOG_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120466EF1a_NEUROD1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120467EF1a_NEUROG1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120468EF1a_NEUROG3_P2A_Hygro_BarcodeBarcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120469EF1a_NRL_P2A_Hygro_BarcodeBarcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120470EF1a_ONECUT1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120471EF1a_OTX2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120472EF1a_PAX7_P2A_Hygro_BarcodeBarcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120473EF1a_POU1F1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120474EF1a_POU5F1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120475EF1a_RUNX1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120476EF1a_SIX1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120477EF1a_SIX2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SIX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120478EF1a_SNAI2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SNAI2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120479EF1a_SOX10_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120480EF1a_SOX2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120481EF1a_SOX3_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120482EF1a_SPI1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120483EF1a_SPIB_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120484EF1a_SPIC_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120485EF1a_SRY_P2A_Hygro_BarcodeBarcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120486EF1a_TBX5_P2A_Hygro_BarcodeBarcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120487EF1a_TFAP2C_P2A_Hygro_BarcodeBarcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120488EF1a_KLF1_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120489EF1a_KLF2_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120490EF1a_KLF3_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120491EF1a_KLF5_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120492EF1a_KLF6_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120493EF1a_KLF7_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120494EF1a_KLF8_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120495EF1a_KLF9_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120496EF1a_KLF10_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120497EF1a_KLF11_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120498EF1a_KLF12_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120499EF1a_KLF13_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120500EF1a_KLF14_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120501EF1a_KLF15_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120502EF1a_KLF16_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120503EF1a_KLF17_P2A_Hygro_BarcodeBarcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120504EF1a_MYC deltaMBI_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120507EF1a_MYC deltab_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120508EF1a_MYC deltaHLH_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120509EF1a_MYC deltaLZ_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120510EF1a_MYC deltaNTD_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120511EF1a_MYC deltaCTD_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120512EF1a_MYC Glu39Ala_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120513EF1a_MYC Thr58Ala_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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120514EF1a_MYC Ser62Ala_P2A_Hygro_BarcodeBarcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
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135003EF1a_mCherry_P2A_HygroLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
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