pLNCX2-mEGFP-TSG101
(Plasmid #116925)

• Purpose: expresses mEGFP-TSG101 in mammalian cells
• Depositing Lab: Sanford Simon
• Publication: Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12211-6. doi: 10.1073/pnas.1321655111. Epub 2014 Aug 6.

Backbone

• Vector backbone: pLNCX2
  (Search Vector Database)
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TSG101
• Species: H. sapiens (human)
• Entrez Gene: TSG101 (a.k.a. TSG10, VPS23)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEGFP (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms:
  • Not Available to Industry