Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #115334)


Item Catalog # Description Quantity Price (USD)
Plasmid 115334 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    silent mutation in position 4050 C to T
  • Entrez Gene
    VPS4A (a.k.a. SKD1, SKD1A, SKD2, VPS4, VPS4-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV
  • 3′ sequencing primer mCherry-FWD
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNCX2-mCherry-VPS4A was a gift from Sanford Simon (Addgene plasmid # 115334 ; ; RRID:Addgene_115334)
  • For your References section:

    Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly. Johnson DS, Bleck M, Simon SM. Elife. 2018 Jul 4;7. pii: 36221. doi: 10.7554/eLife.36221. 36221 [pii] PubMed 29972351