pLNCX2-mCherry-CHMP4B
(Plasmid #116923)

• Purpose: expresses mCherry-CHMP4B in mammalian cells
• Depositing Lab: Sanford Simon
• Publication: Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12211-6. doi: 10.1073/pnas.1321655111. Epub 2014 Aug 6.

Backbone

• Vector backbone: pLNCX2
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CHMP4B
• Species: H. sapiens (human)
• Entrez Gene: CHMP4B (a.k.a. C20orf178, CHMP4A, CTPP3, CTRCT31, SNF7, SNF7-2, Shax1, VPS32B, Vps32-2, dJ553F4.4)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: mCherry-FWD

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry