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pLNCX2-mEGFP-CHMP2A
(Plasmid #115328)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115328 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLNCX2
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CHMP2A
  • Species
    H. sapiens (human)
  • Entrez Gene
    CHMP2A (a.k.a. BC-2, BC2, CHMP2, VPS2, VPS2A)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNCX2-mEGFP-CHMP2A was a gift from Sanford Simon (Addgene plasmid # 115328 ; http://n2t.net/addgene:115328 ; RRID:Addgene_115328)
  • For your References section:

    Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly. Johnson DS, Bleck M, Simon SM. Elife. 2018 Jul 4;7. pii: 36221. doi: 10.7554/eLife.36221. 36221 [pii] PubMed 29972351