pLNCX2-mCherry-CHMP2A
(Plasmid
#115330)
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Purposeexpresses mCherry-CHMP2A in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 115330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLNCX2
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Backbone manufacturerClontech
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCHMP2A
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SpeciesH. sapiens (human)
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Entrez GeneCHMP2A (a.k.a. BC-2, BC2, CHMP2, VPS2, VPS2A)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BspEI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer mCherry-FWD (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLNCX2-mCherry-CHMP2A was a gift from Sanford Simon (Addgene plasmid # 115330 ; http://n2t.net/addgene:115330 ; RRID:Addgene_115330) -
For your References section:
Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly. Johnson DS, Bleck M, Simon SM. Elife. 2018 Jul 4;7. pii: 36221. doi: 10.7554/eLife.36221. 36221 [pii] PubMed 29972351
