pBSSK p85 alpha (delta iSH2)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13432||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep85 alpha (delta iSH2)
Alt namep85 alpha
SpeciesH. sapiens (human)
MutationDominant negative p85 alpha. The p110 alpha binding domain deleted.
Entrez GenePIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA)
/ Fusion Protein
- flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
see author's map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBSSK p85 alpha (delta iSH2) was a gift from Ronald Kahn (Addgene plasmid # 13432 ; http://n2t.net/addgene:13432 ; RRID:Addgene_13432)
For your References section:Phosphoinositide 3-kinase regulatory subunit p85alpha suppresses insulin action via positive regulation of PTEN. Taniguchi CM, Tran TT, Kondo T, Luo J, Ueki K, Cantley LC, Kahn CR. Proc Natl Acad Sci U S A. 2006 Aug 8. 103(32):12093-7. 10.1073/pnas.0604628103 PubMed 16880400
Map uploaded by the depositor.