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pGEX-4T1-14-3-3 epsilon GST
(Plasmid #13279)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13279 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 epsilon
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Ywhae (a.k.a. 14-3-3e)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer pGEX
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Insert from epsilon-MBP cloned into BamHI and SmaI (which is now lost).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1-14-3-3 epsilon GST was a gift from Michael Yaffe (Addgene plasmid # 13279 ; http://n2t.net/addgene:13279 ; RRID:Addgene_13279)
  • For your References section:

    The structural basis for 14-3-3:phosphopeptide binding specificity. Yaffe MB, Rittinger K, Volinia S, Caron PR, Aitken A, Leffers H, Gamblin SJ, Smerdon SJ, Cantley LC. Cell. 1997 Dec 26. 91(7):961-71. 10.1016/S0092-8674(00)80487-0 PubMed 9428519