pGEX-2TK 14-3-3 sigma GST
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11944||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name14-3-3 sigma
SpeciesH. sapiens (human)
Entrez GeneSFN (a.k.a. YWHAS)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI? (unknown if destroyed)
- 5′ sequencing primer pGEX_5
- 3′ sequencing primer pGEX_3 (Common Sequencing Primers)
Uses stop codons of pGEX-2TK rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-2TK 14-3-3 sigma GST was a gift from Michael Yaffe (Addgene plasmid # 11944 ; http://n2t.net/addgene:11944 ; RRID:Addgene_11944)
For your References section:The structural basis for 14-3-3:phosphopeptide binding specificity. Yaffe MB, Rittinger K, Volinia S, Caron PR, Aitken A, Leffers H, Gamblin SJ, Smerdon SJ, Cantley LC. Cell. 1997 Dec 26. 91(7):961-71. 10.1016/S0092-8674(00)80487-0 PubMed 9428519