pGEX-4T1-14-3-3 zeta GST
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13278||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4969
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name14-3-3 zeta
SpeciesB. taurus (bovine)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI? (unknown if destroyed)
- 3′ cloning site BamHI? (unknown if destroyed)
- 5′ sequencing primer pGEX (Common Sequencing Primers)
Insert PCR'd and cloned into pGEX-4T1 (via either EcoRI and/or BamHI?) by Tom Roberts lab.
Uses stop codons of pGEX backbone rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-4T1-14-3-3 zeta GST was a gift from Michael Yaffe (Addgene plasmid # 13278 ; http://n2t.net/addgene:13278 ; RRID:Addgene_13278)
For your References section:The structural basis for 14-3-3:phosphopeptide binding specificity. Yaffe MB, Rittinger K, Volinia S, Caron PR, Aitken A, Leffers H, Gamblin SJ, Smerdon SJ, Cantley LC. Cell. 1997 Dec 26. 91(7):961-71. 10.1016/S0092-8674(00)80487-0 PubMed 9428519