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pGEX-4T2-14-3-3 tau GST
(Plasmid #13281)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 13281 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    14-3-3 tao
  • Alt name
    14-3-3 theta
  • Species
    H. sapiens (human)
  • Entrez Gene
    YWHAQ (a.k.a. 14-3-3, 1C5, HS1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA from Gerd Walz lab.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Uses stop codons of pGEX backbone rather than 14-3-3 and contains a small extra C-terminal extension. This has been corrected in the HA-tagged constructs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T2-14-3-3 tau GST was a gift from Michael Yaffe (Addgene plasmid # 13281 ; ; RRID:Addgene_13281)
  • For your References section:

    The structural basis for 14-3-3:phosphopeptide binding specificity. Yaffe MB, Rittinger K, Volinia S, Caron PR, Aitken A, Leffers H, Gamblin SJ, Smerdon SJ, Cantley LC. Cell. 1997 Dec 26. 91(7):961-71. 10.1016/S0092-8674(00)80487-0 PubMed 9428519