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Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference.
Zhang E, Neugebauer ME, Krasnow NA, Liu DR
Nat Commun. 2024 Feb 24;15(1):1697. doi: 10.1038/s41467-024-45969-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
215819SpCas9-CBE6aExpress CBE6a (with SpCas9) in mammalian cells
215820SpCas9-CBE6bExpress CBE6b (with SpCas9) in mammalian cells
215821SpCas9-CBE6cExpress CBE6c (with SpCas9) in mammalian cells
215822SpCas9-CBE6dExpress CBE6d (with SpCas9) in mammalian cells
215824SpCas9-CBE6a-V106WExpress CBE6a-V106W (with SpCas9) in mammalian cells
215825SpCas9-CBE6b-V106WExpress CBE6b V106W (with SpCas9) in mammalian cells
215826SpCas9-CBE6c-V106WExpress CBE6c V106W (with SpCas9) in mammalian cells
215827SpCas9-CBE6d-V106WExpress CBE6d V106W (with SpCas9) in mammalian cells
215829eNme2C-CBE6aExpress CBE6a (with eNme2-C variant) in mammalian cells
215830eNme2C-CBE6bExpress CBE6b (with eNme2-C variant) in mammalian cells
215831eNme2C-CBE6cExpress CBE6c (with eNme2-C variant) in mammalian cells
215832eNme2C-CBE6dExpress CBE6d (with eNme2-C variant) in mammalian cells
215833SpCas9-CBE6a-IVTTemplate for in vitro transcription of CBE6a (with SpCas9)
215834SpCas9-CBE6b-IVTTemplate for in vitro transcription of CBE6b (with SpCas9)
215835SpCas9-CBE6c-IVTTemplate for in vitro transcription of CBE6c (with SpCas9)
215836SpCas9-CBE6d-IVTTemplate for in vitro transcription of CBE6d (with SpCas9)

Antibodies from Article