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A versatile tool for conditional gene expression and knockdown.
Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
Nat Methods. 2006 Feb . 3(2):109-16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
11642pLVPT-tTR-KRABTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
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11643pLVCT-tTR-KRABTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
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11644pLVET-tTR-KRABTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
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11645pLVU-tTR-KRAB
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11646pLVPT-GDNF-tTR-KRAB
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11647pLVPT-GDNF-rtTR-KRAB-2SM2
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11648pLVPRT-tTR-KRABTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
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11650pLVUTHshGATA1-tTR-KRAB
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11651pLVUT-tTR-KRABTet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation
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11652pLVPT-rtTR-KRAB-2SM2Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
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11653pLVUHshp53
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11654pLVUHshp53-tTR-KRAB
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11777pLVPT-rtTR-KRAB
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11779pLVCT-rtTR-KRAB-2SM2Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation
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