A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.
Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. PubMed Article
Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 157833 | G1333 DddAtox-N–dSpCas9 | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157834 | G1333 DddAtox-C–dSpCas9 | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157835 | G1397 DddAtox-N–dSpCas9 | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157836 | G1397 DddAtox-C–dSpCas9 | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157837 | G1333 DddAtox-N–SaKKH-Cas9(D10A) | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157838 | G1333 DddAtox-C–SaKKH-Cas9(D10A) | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157839 | G1397 DddAtox-N–SaKKH-Cas9(D10A) | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157840 | G1397 DddAtox-C–SaKKH-Cas9(D10A) | expresses split DddAtox-Cas9 construct in mammalian cells |
| 157841 | ND6-DdCBE-right side TALE | expresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells |
| 157842 | ND6-DdCBE-left side TALE | expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells |
| 157843 | ND4-DdCBE-right side TALE | expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells |
| 157844 | ND4-DdCBE- left side TALE | expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells |
| 158092 | ND4 left TALE–G1333 DddAtox C | expresses the right-side ND4 TALE–DddAtox half in mammalian cells |
| 158093 | ND4 left TALE–G1333 DddAtox N | expresses the left-side ND4 TALE–DddAtox half in mammalian cells |
| 158094 | ND4 left TALE–G1397 DddAtox N | expresses the right-side ND4 TALE–DddAtox half in mammalian cells |
| 158095 | ND4 right TALE–G1333 DddAtox C | expresses the right-side ND4 TALE–DddAtox half in mammalian cells |
| 158096 | ND4 right TALE–G1333 DddAtox N | expresses the right-side ND4 TALE–DddAtox half in mammalian cells |
| 158097 | ND4 right TALE–G1397 DddAtox C | expresses the right-side ND4 TALE–DddAtox half in mammalian cells |
| 179631 | DH5alpha::DddI | For expression of toxic DddA |