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A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing.

Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR
Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
157833G1333 DddAtox-N–dSpCas9expresses split DddAtox-Cas9 construct in mammalian cells
157834G1333 DddAtox-C–dSpCas9expresses split DddAtox-Cas9 construct in mammalian cells
157835G1397 DddAtox-N–dSpCas9expresses split DddAtox-Cas9 construct in mammalian cells
157836G1397 DddAtox-C–dSpCas9expresses split DddAtox-Cas9 construct in mammalian cells
157837G1333 DddAtox-N–SaKKH-Cas9(D10A)expresses split DddAtox-Cas9 construct in mammalian cells
157838G1333 DddAtox-C–SaKKH-Cas9(D10A)expresses split DddAtox-Cas9 construct in mammalian cells
157839G1397 DddAtox-N–SaKKH-Cas9(D10A)expresses split DddAtox-Cas9 construct in mammalian cells
157840G1397 DddAtox-C–SaKKH-Cas9(D10A)expresses split DddAtox-Cas9 construct in mammalian cells
157841ND6-DdCBE-right side TALEexpresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells
157842ND6-DdCBE-left side TALEexpresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells
157843ND4-DdCBE-right side TALEexpresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells
157844ND4-DdCBE- left side TALEexpresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells
158092ND4 left TALE–G1333 DddAtox Cexpresses the right-side ND4 TALE–DddAtox half in mammalian cells
158093ND4 left TALE–G1333 DddAtox Nexpresses the left-side ND4 TALE–DddAtox half in mammalian cells
158094ND4 left TALE–G1397 DddAtox Nexpresses the right-side ND4 TALE–DddAtox half in mammalian cells
158095ND4 right TALE–G1333 DddAtox Cexpresses the right-side ND4 TALE–DddAtox half in mammalian cells
158096ND4 right TALE–G1333 DddAtox Nexpresses the right-side ND4 TALE–DddAtox half in mammalian cells
158097ND4 right TALE–G1397 DddAtox Cexpresses the right-side ND4 TALE–DddAtox half in mammalian cells
179631DH5alpha::DddIFor expression of toxic DddA

Antibodies from Article