DH5alpha::DddI
(Bacterial strain
#179631)
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PurposeFor expression of toxic DddA
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 179631 | Bacteria in agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonen/a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 15 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha::DddI
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedddI
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Genotype: F– φ80lacZΔM15 Δ(lacZYAargF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1, with chromosomally integrated dddI
PCR verification primers (expected product: ~450 bp): dddI-F: 5'-ATTCGAGCTCACGGGAGGAA-3' and dddI-R: 5'-CCTGCAGGTCGACCTCATTT-3'
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DH5alpha::DddI was a gift from David Liu (Addgene plasmid # 179631) -
For your References section:
A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. 10.1038/s41586-020-2477-4 PubMed 32641830