PurposeExpresses tTA2 under the CAG promoter in mammalian cells.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104102||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)747
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-tTA was a gift from Takeshi Imai (Addgene plasmid # 104102 ; http://n2t.net/addgene:104102 ; RRID:Addgene_104102)
For your References section:Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags. Sakaguchi R, Leiwe MN, Imai T. Elife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350. 10.7554/eLife.40350 PubMed 30454553