Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBS-TRE-mTurquoise2-WPRE
(Plasmid #104103)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104103 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Modifications to backbone
    An Xho-Xho fragment containing TRE-SV40 poly A was transferred from pTRE-Tight (Clontech).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTurquoise2
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The mTurquoise2 gene was cloned from pmTurquoise2-N1 (Addgene #60561 from Dr. D. Gadella).
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-TRE-mTurquoise2-WPRE was a gift from Takeshi Imai (Addgene plasmid # 104103 ; http://n2t.net/addgene:104103 ; RRID:Addgene_104103)
  • For your References section:

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags. Sakaguchi R, Leiwe MN, Imai T. Elife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350. 10.7554/eLife.40350 PubMed 30454553