Skip to main content
Addgene

pET28a-SpySrtA
(Plasmid #51139)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 51139 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S.pyogenes SrtA
  • Alt name
    Sortase A
  • Species
    S.pyogenes
  • Insert Size (bp)
    510
  • Mutation
    Deleted amino acids 1-80
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    original construct from Mark Banfield's lab
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SpySrtA was a gift from Hidde Ploegh (Addgene plasmid # 51139 ; http://n2t.net/addgene:51139 ; RRID:Addgene_51139)

  • For your References section:

    Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions. Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, Ploegh HL. Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub 2013 Aug 29. 10.1038/nprot.2013.101 PubMed 23989673
Related items:
Commonly requested with: