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pet30b-7M SrtA
(Plasmid #51141)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51141 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET30b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5421
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S.aureus SrtA 7M
  • Alt name
    Calcium independent sortase A
  • Alt name
    Heptamutant Sortase A
  • Species
    S.aureus
  • Insert Size (bp)
    471
  • Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from David Liu's pentamutant sortase, includes two additional amino acid mutations which rendered it calcium independent: E105K, E108Q
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet30b-7M SrtA was a gift from Hidde Ploegh (Addgene plasmid # 51141 ; http://n2t.net/addgene:51141 ; RRID:Addgene_51141)