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pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
(Plasmid #52053)


Item Catalog # Description Quantity Price (USD)
Plasmid 52053 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2870
  • Total vector size (bp) 4279
  • Modifications to backbone
    tetR/pLtetO promoter system added
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
  • Promoter tetR/pLtetO

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer Tet-R
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To clone new genes for your proteins of interest into the pCRT7 NT vector provided, use 5’KpnI--‐3’HindIII restriction sites.
See Lutz and Bujard 1997 (PMID 9092630) for information on the tetR/pLtetO promoter system used for inducible expression in the pCRT7 NT vector.
The phosphoproteins (or sfGFP) expressed via the pCRT7 pLtetO/tetR plasmid is induced with 100 ng/mL anhydrotetracycline.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP was a gift from Jesse Rinehart (Addgene plasmid # 52053 ; ; RRID:Addgene_52053)