pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52053||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2870
- Total vector size (bp) 4279
Modifications to backbonetetR/pLtetO promoter system added
Vector typeBacterial Expression
Growth in Bacteria
- Promoter tetR/pLtetO
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Tet-R
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
To clone new genes for your proteins of interest into the pCRT7 NT vector provided, use 5’KpnI--‐3’HindIII restriction sites.
See Lutz and Bujard 1997 (PMID 9092630) for information on the tetR/pLtetO promoter system used for inducible expression in the pCRT7 NT vector.
The phosphoproteins (or sfGFP) expressed via the pCRT7 pLtetO/tetR plasmid is induced with 100 ng/mL anhydrotetracycline.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP was a gift from Jesse Rinehart (Addgene plasmid # 52053 ; http://n2t.net/addgene:52053 ; RRID:Addgene_52053)