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SepOTSλ (recommended)
(Plasmid #68292)


Item Catalog # Description Quantity Price (USD)
Plasmid 68292 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    pKD is derived from pKK223-3 (Pharmacia). This plasmid encodes a variant of the phosphoserine orthogonal translation system. Another plasmid encoding a protein of interest with TAG at the proper position is needed for recombinant phosphoprotein expression. See article and supplementary information for cloning information.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    low Kan
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    This plasmid is slow growing and may take up to 24 hours to produce colonies. Note: a few colonies may grow much faster than the rest. These colonies often do not contain the full, intact construct (as though part of the plasmid is chopped out). We recommend isolating the plasmid from any of the (majority) colonies that grow much more slowly.
  • Copy number


  • Gene/Insert name
    SepRS9/EFSep21, 4xtRNA-Sep-A37
  • Species

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

See supplementary information accompanying article for additional cloning information. We recommend introducing this plasmid into a recoded strain of E. coli (no genomic TAG codons) for high-efficiency phoshoprotein expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SepOTSλ (recommended) was a gift from Jesse Rinehart (Addgene plasmid # 68292 ; ; RRID:Addgene_68292)
  • For your References section:

    A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. 10.1038/ncomms9130 PubMed 26350500