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PCRT7 tetR pLtetO MBP-MEK1 XX Amp
(Plasmid #53225)


Item Catalog # Description Quantity Price (USD)
Plasmid 53225 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Rinehart lab, Addgene plasmid 52053
  • Total vector size (bp) 6001
  • Vector type
    Bacterial Expression ; phosphoprotein synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    mitogen-activated protein kinase kinase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Codon optimized for E.coli, both codons for Ser218 and Ser222 were changed to amber codons
  • Entrez Gene
    MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
  • Promoter pLtetO
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCRT7 tetR pLtetO MBP-MEK1 XX Amp was a gift from Jesse Rinehart (Addgene plasmid # 53225 ; ; RRID:Addgene_53225)