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A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
68283SepOTSβ Phosphoserine orthogonal translation system
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68284SepOTSγPhosphoserine orthogonal translation system
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68285SepOTSδPhosphoserine orthogonal translation system
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68286SepOTSεPhosphoserine orthogonal translation system
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68287SepOTSζPhosphoserine orthogonal translation system
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68288SepOTSηPhosphoserine orthogonal translation system
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68289SepOTSθPhosphoserine orthogonal translation system
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68290SepOTSιPhosphoserine orthogonal translation system
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68291SepOTSκPhosphoserine orthogonal translation system
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68292SepOTSλ (recommended)Phosphoserine orthogonal translation system (preferred variant)
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68294SepOTSνPhosphoserine orthogonal translation system
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68295GFP E17TAGE17TAG GFP reporter
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68296GFP S2TAGS2TAG GFP reporter
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68297GFP Q157TAGQ157TAG GFP reporter
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68298GFP S2TAG/E17TAGS2TAG/E17TAG GFP reporter
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68299GFP E17TAG/Q157TAGE17/Q157 TAG GFP reporter
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68300MBP-MEK1N-terminal MBP tagged human MEK1 gene for bacterial expression
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68301MBP-MEK1 S218TAGN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression
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68302MBP-MEK1 S222TAGN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression
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68305Beta lactamase S68TAGBeta lactamase S68TAG gene
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68306Strain: C321.ΔAGenomically recoded strain of E. coli (no genomic TAG)
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68307SupDPlasmid encoding tRNA-SupD for serine amber suppression
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69118E17TAG GFP zeo resistanceFor E17TAG GFP reporter expression in non-zeocin resistant strains
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