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A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
68283SepOTSβ Phosphoserine orthogonal translation system
68284SepOTSγPhosphoserine orthogonal translation system
68285SepOTSδPhosphoserine orthogonal translation system
68286SepOTSεPhosphoserine orthogonal translation system
68287SepOTSζPhosphoserine orthogonal translation system
68288SepOTSηPhosphoserine orthogonal translation system
68289SepOTSθPhosphoserine orthogonal translation system
68290SepOTSιPhosphoserine orthogonal translation system
68291SepOTSκPhosphoserine orthogonal translation system
68292SepOTSλPhosphoserine orthogonal translation system (preferred variant)
68294SepOTSνPhosphoserine orthogonal translation system
68295GFP E17TAGE17TAG GFP reporter
68296GFP S2TAGS2TAG GFP reporter
68297GFP Q157TAGQ157TAG GFP reporter
68298GFP S2TAG/E17TAGS2TAG/E17TAG GFP reporter
68299GFP E17TAG/Q157TAGE17/Q157 TAG GFP reporter
68300MBP-MEK1N-terminal MBP tagged human MEK1 gene for bacterial expression
68301MBP-MEK1 S218TAGN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression
68302MBP-MEK1 S222TAGN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression
68305Beta lactamase S68TAGBeta lactamase S68TAG gene
68306Strain: C321.ΔAGenomically recoded strain of E. coli (no genomic TAG)
69118E17TAG GFP zeo resistanceFor E17TAG GFP reporter expression in non-zeocin resistant strains
198598SupD v2Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

Antibodies from Article