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Addgene

SupD v2
(Plasmid #198598)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 198598 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKD is derived from pKK223-3 (Pharmacia).
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 4926
  • Modifications to backbone
    2 copies of SupD locus
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SupD
  • Alt name
    Serine supresor tRNA (UAG-Amber)
  • Species
    Synthetic
  • Insert Size (bp)
    432

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid encodes a serine amber (TAG) suppressor tRNA. Another plasmid encoding a protein of interest with TAG at the proper position is needed for recombinant protein expression. See article and supplementary information for cloning information. This is an improved version of Plasmid 68307: SupD.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SupD v2 was a gift from Jesse Rinehart (Addgene plasmid # 198598 ; http://n2t.net/addgene:198598 ; RRID:Addgene_198598)
  • For your References section:

    A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J. Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130. 10.1038/ncomms9130 PubMed 26350500