pSerOTS-C1* (V70)
(Plasmid #188537)

• Purpose: Phosphoserine incorporation into recombinant proteins
• Depositing Lab: Jesse Rinehart
• Publication: et al BioRxiv 2021, 2021.05.20.444985

Backbone

• Vector backbone: pKD-pSerRS-Ef-pSer
  (Search Vector Database)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pSerRS
• Species: Methanococcus maripaludis
• Insert Size (bp): 1620
• Mutation: mutations resulting from laboratory evolution
• Promoter: glnS*

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: Ef-pSer
• Species: Methanococcus maripaludis
• Insert Size (bp): 1185
• Mutation: mutations resulting from laboratory evolution

Gene/Insert 3

• Gene/Insert name: tRNApSer
• Species: Methanococcus maripaludis
• Insert Size (bp): 220

Resource Information

• Supplemental Documents:
  • pSerOTS-C1_ (V70).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry