PurposeMG1655-C321 -- All 321 UAG codons changed to UAA
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||192872||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Bleocin(Zeocin), 50 μg/mL
Growth instructionsThis strain can be grown in standard LB but must be supplemented with d-biotin and Serine when grown in minimal media.
Gene/Insert nameMG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
MG1655-C321 -- All 321 UAG codons changed to UAA.
mutS+ -- Restores normal spontaneous mutation frequency.
(λ-) – Deleted Lambda Phage operon to allow growth at 37C.
Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph.
ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons.
ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:rEcoli XpS was a gift from Jesse Rinehart (Addgene plasmid # 192872)
For your References section:A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics. Gassaway BM, Li J, Rad R, Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP. Nat Methods. 2022 Oct 24. pii: 10.1038/s41592-022-01638-5. doi: 10.1038/s41592-022-01638-5. 10.1038/s41592-022-01638-5 PubMed 36280721