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Addgene

pB-CAG-FLEX-F14F15-BFP-(mCherry)'
(Plasmid #201831)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201831 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pB-CAG
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 8924
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CAG
  • Alt name
    CAG promoter
  • Species
    Synthetic
  • Insert Size (bp)
    1699
  • Promoter CAG

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    BFP
  • Alt name
    mTagBFP2
  • Species
    Synthetic

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-CAG-FLEX-F14F15-BFP-(mCherry)' was a gift from Ian Wickersham (Addgene plasmid # 201831 ; http://n2t.net/addgene:201831 ; RRID:Addgene_201831)
  • For your References section:

    Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency. Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou Y, Feng G, Wickersham IR. Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 10.1016/j.crmeth.2023.100644. 10.1016/j.crmeth.2023.100644 PubMed 37989085