Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency.
Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou Y, Feng G, Wickersham IR
Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 10.1016/j.crmeth.2023.100644. PubMed Article
Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou Y, Feng G, Wickersham IR
Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 10.1016/j.crmeth.2023.100644. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182964 | pRV∆L-5Cre | The 3rd generation of rabies vector expressing Cre recombinase |
| 182966 | pRV∆L-5tTA | The 3rd generation of rabies vector expressing a tetracycline-controlled transactivator (tTA) |
| 182967 | pB-TREtight-EGFP | For making cell lines for titering pRV∆L-5tTA virus |
| 201831 | pB-CAG-FLEX-F14F15-BFP-(mCherry)' | piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase |