Jesse Rinehart Lab: Rinehart Lab Phosphoprotein synthesis components

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
52053	pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP	Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
52054	pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)	Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)