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pRP_ASC-LmCerulean
(Plasmid #41840)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41840 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRP_LmCerulean
  • Backbone size w/o insert (bp) 7332
  • Total vector size (bp) 7911
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    apoptosis-associated speck-like protein containing CARD
  • Alt name
    ASC; PYCARD; TMS; TMS1; CARD5; TMS-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    585
  • Mutation
    Stop Codon removed
  • GenBank ID
    NM_013258
  • Entrez Gene
    PYCARD (a.k.a. ASC, CARD5, TMS, TMS-1, TMS1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCerulean (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer AAGCAGAGCTCGTTTAGTGAA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRP_ASC-LmCerulean was a gift from Eicke Latz (Addgene plasmid # 41840 ; http://n2t.net/addgene:41840 ; RRID:Addgene_41840)
  • For your References section:

    ASC speck formation as a readout for inflammasome activation. Stutz A, Horvath GL, Monks BG, Latz E. Methods Mol Biol. 2013;1040:91-101. doi: 10.1007/978-1-62703-523-1_8. 10.1007/978-1-62703-523-1_8 PubMed 23852599