PurposeE. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||124700||Bacteria in agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)DAP (400 µg/ml)
Growth Strain(s)This is a strain.
Growth instructionsGrow in liquid LB medium or LB agar plates supplemented with 400 μg/ml 2,6-diaminopimelic acid (DAP) to satisfy the strain’s DAP auxotrophy due the Δasd mutation.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Δasd thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA; integrated RP4-2 Tcr::Mu ΔaphA (λpir+)
Strain RHO3 was derived from SM10(λpir+) by deletion of the chromosomal asd gene and the aphA kanamycin resistance determinant located on the chromosomally integrated RP4-2 element (López et al. 2009. Appl. Env. Microbiol. 75:6496-6503).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RHO3 was a gift from Herbert Schweizer (Addgene plasmid # 124700)
For your References section:Versatile dual-technology system for markerless allele replacement in Burkholderia pseudomallei. Lopez CM, Rholl DA, Trunck LA, Schweizer HP. Appl Environ Microbiol. 2009 Oct;75(20):6496-503. doi: 10.1128/AEM.01669-09. Epub 2009 Aug 21. 10.1128/AEM.01669-09 PubMed 19700544