RHO3
(Bacterial strain #124700)

• Purpose: E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.
• Depositing Lab: Herbert Schweizer
• Publication: Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):6496-503. doi: 10.1128/AEM.01669-09. Epub 2009 Aug 21.

Backbone

• Vector backbone: none

Growth in Bacteria

• Bacterial Resistance(s): DAP (400 µg/ml)
• Growth Temperature: 37°C
• Growth Strain(s): This is a strain.
• Growth instructions: Grow in liquid LB medium or LB agar plates supplemented with 400 μg/ml 2,6-diaminopimelic acid (DAP) to satisfy the strain’s DAP auxotrophy due the Δasd mutation.
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • RHO3 strain verification

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genotype:
Δasd thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA; integrated RP4-2 Tcr::Mu ΔaphA (λpir+)

Strain RHO3 was derived from SM10(λpir+) by deletion of the chromosomal asd gene and the aphA kanamycin resistance determinant located on the chromosomally integrated RP4-2 element (López et al. 2009. Appl. Env. Microbiol. 75:6496-6503).