166,485 results

1. pJExpress414_GmPOPB-S577A

   Plasmid

   #92235

   Purpose

   Express S577A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-S577A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Serine 577 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-li-p33_RS-CLIP

   Plasmid

   #104634

   Purpose

   Human Invariant chain with a CLIP replacement with in frame restriction sites (KpnI/XhoI)

   Depositor

   Sébastien Walchli

   Article

   Mensali et al Oncoimmunology. 2019 Jan 11;8(3):1558663

   Insert

   modified Invariant chain p33 (CD74 Human)
   

   Use

   Entry vector for gateway system

   Tags

   none

   Expression

   Mutation

   CLIP domain was replaced by 2 restriction sites

   Promoter

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α-DIO-CFL-SN

   Plasmid

   #181740

   Purpose

   AAV vector for expressing CFL-SN

   Depositor

   Yasunori Hayashi

   Article

   Goto et al Science. 2021 Nov 12;374(6569):857-863.

   Insert

   CFL-SN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSB219

   Plasmid

   #164125

   Purpose

   p15A backbone with PhlF binding sites

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6p-2-EWSR1

   Plasmid

   #46384

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. PX393 Gluconacetobacter diazotrophicus Cas9

   Plasmid

   #68330

   Purpose

   Gluconacetobacter diazotrophicus Cas9

   Depositor

   Feng Zhang

   Insert

   GdCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. phage UbiC G3BP1-GFP-GFP

   Plasmid

   #119950

   Purpose

   Lentiviral vector expressing G3BP1-GFP fusion protein

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbiC

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJExpress414_GmPOPB-D661A

   Plasmid

   #92236

   Purpose

   Express D661A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-D661A mutant
   

   Use

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Aspartate 661 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-Anillin

   Plasmid

   #68027

   Purpose

   Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

   Depositor

   Michael Glotzer

   Article

   Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

   Insert

   Anillin (ANLN Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   silent mutations at 798 5'-TGCC TCTTTGAATAAA…

   Promoter

   CMV

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-NES-jRCaMP1a

    Plasmid

    #61562

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRCaMP1a
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNUTN6His Y426F/Y517F hTFNG

    Plasmid

    #70084

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNUT N6His Y95F/Y188F hTFNG

    Plasmid

    #69601

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe

    Depositor

    Ross MacGillivray, Anne Mason

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. iRFP-PH-PLCdelta1

    Plasmid

    #66841

    Purpose

    expression of near-infrared PI(4,5)P2 biosensor

    Depositor

    Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    PH-PLCdelta1
    

    Use

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-CMV-Galpha-12 ONE-GO

    Plasmid

    #189739

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-12 ONE-GO
    GNA12 (GNA12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid

    #226429

    Purpose

    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

    Depositor

    Alon Goren

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Hia5-OPT1

    Plasmid

    #231968

    Purpose

    Expresses DNA adenine N6-methyltransferase Hia5 in Escherichia coli (E.coli) T7 Express lysY/Iq

    Depositor

    Schahram Akbarian

    Article

    Peter et al Cell Rep Methods. 2024 Nov 25:100911. do

    Insert

    DNA adenine N6-methyltransferase Hia5
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpRY_ATH51-IP

    Plasmid

    #235991

    Purpose

    Expresses SpRY_ATH51 in mammalian cells

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    SpRY_ATH51
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    ectopic insertion of 7 amino acids of PID turn-he…

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR-miRFP670

    Plasmid

    #228612

    Purpose

    Mammalian expression of miRFP670, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    miRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-6xHis [N144/14-rat IgG2a] - Chimeric

    Plasmid

    #227037

    Purpose

    Mammalian expression plasmid of anti-6xHis rat IgG2a R-mAb. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-6xHis recombinant mouse monoclonal antibody.
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR-mTagBFP2

    Plasmid

    #228596

    Purpose

    Mammalian expression of mTagBFP2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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