High-yield vesicle-packaged recombinant protein production from E. coli.
Eastwood TA, Baker K, Streather BR, Allen N, Wang L, Botchway SW, Brown IR, Hiscock JR, Lennon C, Mulvihill DP
Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27. PubMed Article
Eastwood TA, Baker K, Streather BR, Allen N, Wang L, Botchway SW, Brown IR, Hiscock JR, Lennon C, Mulvihill DP
Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182386 | pRSFDuet-1_VNp-His6 | Bacterial expression of Vesicle Nucleating peptide |
| 182387 | pRSFDuet-1_VNp-mNeongreen | Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion |
| 182388 | pRSFDuet-1_VNp6-mNeongreen | Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion |
| 182389 | pRSFDuet-1_VNp15-mNeongreen | Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion |
| 182390 | pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry | Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions |
| 182391 | pETDuet-1_VNp-mNeongreen_CydAB-mCherry | Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions |
| 182392 | pRSFDuet-1_VNp-LZ-mNeongreen | Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion |
| 182393 | pRSFDuet-1_VNp-LZ_CydAB-mNeongreen | Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions |
| 182395 | pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct) | Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions |
| 182396 | pRSFDuet-1_DARPinOFF7 | Bacterial expression of DARPinOFF7 |
| 182397 | pRSFDuet-1_VNp-DARPinOFF7 | Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion |
| 182398 | pRSFDuet-1_VNp6-DARPinOFF7 | Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion |
| 182399 | pRSFDuet-1_VNp15-DARPinOFF7 | Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion |
| 182400 | pRSFDuet-1_Uricase | Bacterial expression of uricase |
| 182401 | pRSFDuet-1_VNp-Uricase | Bacterial expression of Vesicle Nucleating peptide-uricase fusion |
| 182402 | pRSFDuet-1_StefinA | Bacterial expression of stefinA |
| 182403 | pRSFDuet-1_VNp-StefinA | Bacterial expression of Vesicle Nucleating peptide-stefinA fusion |
| 182404 | pRSFDuet-1_VNp6-StefinA | Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion |
| 182405 | pRSFDuet-1_VNp15-StefinA | Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion |
| 182406 | pRSFDuet-1_FGF21 | Bacterial expression of fgf21 |
| 182407 | pRSFDuet-1_VNp-FGF21 | Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion |
| 182408 | pRSFDuet-1_DNAseI | Bacterial expression of DNaseI |
| 182409 | pRSFDuet-1_VNp-DNAseI | Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion |
| 182410 | pRSFDuet-1_hGH | Bacterial expression of human growth hormone |
| 182411 | pRSFDuet-1_VNp-hGH | Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion |
| 182412 | pRSFDuet-1_VNp-LZ_VNp-LZ-hGH | Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion |
| 182413 | pRSFDuet-1_mNeongreen | Bacterial expression of mNeongreen |
| 182414 | pRSFDuet-1_mNeongreen-DARPinOFF7 | Bacterial expression of mNeongreen-DARPinOFF7 fusion |
| 182415 | pRSFDuet-1_VNp-mNeongreen-DARPinOFF7 | Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion |
| 182416 | pRSFDuet-1_mNeongreen-Uricase | Bacterial expression of mNeongreen-uricase fusion |
| 182417 | pRSFDuet-1_VNp-mNeongreen-Uricase | Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion |
| 182418 | pRSFDuet-1_mNeongreen-StefinA | Bacterial expression of mNeongreen-stefinA fusion |
| 182419 | pRSFDuet-1_VNp-mNeongreen-StefinA | Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion |
| 182420 | pRSFDuet-1_mNeongreen-Etanercept | Bacterial expression of mNeongreen-Etanercept fusion |
| 182421 | pRSFDuet-1_VNp-mNeongreen-Etanercept | Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion |
| 182422 | pRSFDuet-1_mNeongreen-Erythropoietin | Bacterial expression of mNeongreen-Erythropoietin fusion |
| 182423 | pRSFDuet-1_VNp-mNeongreen-Erythropoietin | Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion |
| 182424 | pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept | Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins |
| 182425 | pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH | Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins |