Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept
(Plasmid #182424)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 182424 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSFDUET-1
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VNp-LZ-mNeongreen-Etanercept
  • Species
    H. sapiens (human)
  • GenBank ID
    UniProtKB: P20333
  • Entrez Gene
    TNFRSF1B (a.k.a. CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR)
  • Promoter T7
  • Tag / Fusion Protein
    • Vesicle Nucleating peptide (VNp), Leucine Zipper & mNeongreen (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Gene/Insert 2

  • Gene/Insert name
    VNp-LZ
  • Tag / Fusion Protein
    • Vesicle Nucleating peptide (VNp),

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept was a gift from Dan Mulvihill (Addgene plasmid # 182424 ; http://n2t.net/addgene:182424 ; RRID:Addgene_182424)
  • For your References section:

    High-yield vesicle-packaged recombinant protein production from E. coli. Eastwood TA, Baker K, Streather BR, Allen N, Wang L, Botchway SW, Brown IR, Hiscock JR, Lennon C, Mulvihill DP. Cell Rep Methods. 2023 Feb 2;3(2):100396. doi: 10.1016/j.crmeth.2023.100396. eCollection 2023 Feb 27. 10.1016/j.crmeth.2023.100396 PubMed 36936078