166,818 results

1. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER-scramble

   Plasmid

   #118349

   Purpose

   This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   scrambled shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CeNL_pRSETb

   Plasmid

   #89510

   Purpose

   Cyan color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Use

   Tags

   His X 6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-PVX

   Plasmid

   #213040

   Purpose

   PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-CMV-Luc2-IRES-ZsGreen-W

   Plasmid

   #164432

   Purpose

   Lentiviral vector backbone encoding firefly luciferase and ZsGreen

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Inserts

   Luciferase
   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. murine TCR OTII-2A.pMIG II

   Plasmid

   #52112

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   P2A (from porcine teschovirus-1)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTNS1

   Plasmid

   #64967

   Purpose

   Tn7 transposase expression

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   tnsABCD
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 23, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. CMV::SypHy A4

   Plasmid

   #24478

   Depositor

   Leon Lagnado

   Article

   Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

   Insert

   SypHy (Syp Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-S5E2-GFP-fGFP (AAV PHP.eB)

   Viral Prep

   #135631-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-GFP-fGFP (#135631). In addition to the viral particles, you will also receive purified pAAV-S5E2-GFP-fGFP plasmid DNA. Bicistronic expression of GFP and membrane-targeted GFP under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Promoter

   Tags

   EGFP

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid

    #213031

    Purpose

    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

    Depositor

    Donna Romero, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    

    Use

    Luciferase

    Tags

    Firefly luciferase antisense intron (3' UTR)

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE bidirectional tet-on

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138151

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Green Glifon600

    Plasmid

    #126207

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon600
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p10xUAS-luc2

    Plasmid

    #173006

    Purpose

    firefly luciferase reporter vector with 10x clustered UAS binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    10xUAS-luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

    Pooled Library

    #84003

    Purpose

    Mouse CRISPRa Pooled Library targeting membrane proteins

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)

    Viral Prep

    #171027-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Promoter

    EF1a

    Tags

    tdTomato

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX_EFS-Cre_ppt-del

    Plasmid

    #162073

    Purpose

    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EFS (part of insert)

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA Myc DBC1

    Plasmid

    #35096

    Depositor

    Osamu Hiraike

    Article

    Hiraike et al Br J Cancer. 2010 Mar 16;102(6):1061-7.

    Insert

    deleted in breast cancer 1 (CCAR2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. dTomato-2xrGBD

    Plasmid

    #129625

    Purpose

    Visualization of active RhoA

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    dTomato-2xrGBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag-Tet1 CD

    Plasmid

    #70129

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain.

    Promoter

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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