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Addgene

pFRT/FLAG/HA-DEST PUM2
(Plasmid #40292)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40292 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pFRT/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5372
  • Total vector size (bp) 8567
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pumilio homolog 2
  • Alt name
    PUM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3195
  • GenBank ID
    NM_015317
  • Entrez Gene
    PUM2 (a.k.a. PUMH2, PUML2)
  • Tag / Fusion Protein
    • FLAG-HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are some cloning scars flanking the insert (see Addgene sequence), but they will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/FLAG/HA-DEST PUM2 was a gift from Thomas Tuschl (Addgene plasmid # 40292 ; http://n2t.net/addgene:40292 ; RRID:Addgene_40292)
  • For your References section:

    Transcriptome-wide identification of RNA-binding protein and microRNA target sites by PAR-CLIP. Hafner M, Landthaler M, Burger L, Khorshid M, Hausser J, Berninger P, Rothballer A, Ascano M Jr, Jungkamp AC, Munschauer M, Ulrich A, Wardle GS, Dewell S, Zavolan M, Tuschl T. Cell. 2010 Apr 2;141(1):129-41. 10.1016/j.cell.2010.03.009 PubMed 20371350