178,785 results
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Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway CloningExpressionMammalianPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-TO-hNIL-BSD-mApple
Plasmid#124230PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SrtA-PDGFR Tm
Plasmid#121168PurposepMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR TmDepositorInsertSrtA-PDGFR
UseRetroviralTagsTomato and flagExpressionMammalianAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMAL-CellTag-V3
Pooled library#115645PurposeViral barcoding library to combinatorially index cells for single-cell analysis of clonal dynamicsDepositorHas serviceConcentrated Lentiviral PrepExpressionMammalianSpeciesSyntheticUseLentiviralAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLNCX2-mEGFP-VPS4A
Plasmid#116924Purposeexpresses mEGFP-VPS4A in mammalian cellsDepositorInsertVPS4A (VPS4A Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationsilent mutation in position 4059 C to TPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR CD19-mMegf10 CAR
Plasmid#113013Purposefor production of lentivirus encoding the CD19 CAR-Pmegf10DepositorUseLentiviralTagsGFPExpressionMammalianAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKC-4.04
Plasmid#112085PurposeControl for NMD sensor: pCMV-3XFLAG-Fluc-betaglobinDepositorInsert3XFLAG firefly luciferase-beta-globin (HBB Mouse, Human, Photinus pyralis)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti6.3/TO/CD9-GFP
Plasmid#104402PurposeExpresses CD9 with mEmerald tag (NOT GFP) at the N terminalDepositorAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LLP252 pEF1a-NLS-scFvGCN4-DNMT3a
Plasmid#100941PurposeSingle chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tagDepositorInsertscFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
Tags1xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CWB-cyan pre-eGRASP(p32)
Plasmid#111579PurposeAn AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertcyan pre-eGRASP(p32)
UseAAVPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDawn-sfGFP
Plasmid#107741PurposeExpresses superfolder GFP under the control of a blue-light transcriptional regulatorDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterpLambdaAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-Archon1-KGC-EGFP-ER2-WPRE
Plasmid#108423PurposeArchon1 fluorescent voltage reporter in mammalian expression plasmidDepositorInsertArchon1-KGC-EGFP-ER2
ExpressionMammalianPromoterCAGAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C321.ΔA.M9adapted
Bacterial strain#98568PurposeRecoded strain C321.ΔA adaptively evolved for 1000+ generations in M9 minimal media + 1% glucose and biotinDepositorBacterial resistanceAmpicillinAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-jGCaMP7b-WPRE
Plasmid#104489PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas serviceAAV Retrograde and AAV1InsertjGCaMP7b
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 Flag-HIF1B (#930)
Plasmid#99916Purposemammalian expressionDepositorAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)
Viral prep#92379-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .DepositorAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
TagsHisExpressionBacterialPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_PIK3CA_p.H1047R
Plasmid#82824PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by