166,485 results

1. eGFP STAT1 WT

   Plasmid

   #12301

   Depositor

   Alan Perantoni

   Article

   Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetOn-CLIMP63-mCherry-eGFP

   Plasmid

   #139871

   Purpose

   Dox-inducible lentiviral expression of human CLIMP63

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CLIMP63 (CKAP4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mutation

   Promoter

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

   Plasmid

   #174081

   Purpose

   Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

   Depositor

   Ryan Mehl

   Article

   Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

   Insert

   NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
   

   Use

   Tags

   NES-Flag

   Expression

   Mammalian

   Mutation

   L270G, Y271L, N311G, C313A

   Promoter

   CMV

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV0328

   Plasmid

   #133489

   Purpose

   Expression of nucleo-cytoplasmic mCherry

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMPRA1-CMV-Clover

   Plasmid

   #53538

   Purpose

   Fluorescent reporter control for pMPRA1

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   Clover GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SauCas9 EMX1-pegRNA

   Plasmid

   #169857

   Purpose

   SauCas9 pegRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-AcGFP-Sec61-IRES-Blast

   Plasmid

   #154009

   Purpose

   Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   acGFP-Sec61 (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPT-nlsLacZ

   Plasmid

   #12261

   Depositor

   Didier Trono

   Insert

   LacZ
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Reb_79 aka PAS620

   Plasmid

   #71225

   Purpose

   Reb_1 with RebA(Q91R)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA( G53A, Q91R)

   Promoter

   Available Since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJMD14

    Plasmid

    #138654

    Purpose

    phiOZJ integrating Streptomyces expression vector, intact RDF

    Depositor

    Joshua Blodgett

    Article

    Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PermE* variant sequence from pDA1652

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid

    #70085

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2

    Insert

    mutated human serum transferrin (TF Human)
    

    Use

    Tags

    Hexa His tag and N-terminal signal peptide, 4 aa …

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. NM R2E2 GFP

    Plasmid

    #1208

    Depositor

    Susan Lindquist

    Article

    Liu et al Nature 1999 Aug 5;400(6744):573-6.

    Insert

    sup35 NM domain (SUP35 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    2 additional copies of second oligopeptide repeat…

    Promoter

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCi-mB2m-sgRNA

    Plasmid

    #154090

    Purpose

    pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. L304-EGFP-Tubulin-WT

    Plasmid

    #64060

    Purpose

    EGFP fused at the N-terminus of tubulin; lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

    Insert

    tubulin
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcdna DSG-2 Tailless

    Plasmid

    #89466

    Purpose

    DSG-2 tailless control

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mc2 mNeon

    Plasmid

    #206218

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe EGFR(L858R/T790M)

    Plasmid

    #32073

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L858R/T790M

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR- flag-Lag16-iCAM-1-Tether

    Plasmid

    #205196

    Purpose

    This vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 Tether and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid

    #192786

    Purpose

    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    IFNGR1 (IFNGR1 Human)
    

    Use

    Tags

    Ig k-chain leader sequence and mXFPm

    Expression

    Mammalian

    Mutation

    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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