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(Plasmid #70145)


Item Catalog # Description Quantity Price (USD)
Plasmid 70145 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7283
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    kinesin family member 23
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    KIF23 (a.k.a. CHO1, KNSL5, MKLP-1, MKLP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Note that there are two silent mutations at position 2682 and 4212. The former is an artificial SalI site and the latter a polymorphism (I think). They also have Gly (GGG) artificially inserted between the starting methionine and the lysine 2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-MKLP1 was a gift from Masanori Mishima (Addgene plasmid # 70145 ; ; RRID:Addgene_70145)
  • For your References section:

    Aurora B and 14-3-3 coordinately regulate clustering of centralspindlin during cytokinesis. Douglas ME, Davies T, Joseph N, Mishima M. Curr Biol. 2010 May 25;20(10):927-33. doi: 10.1016/j.cub.2010.03.055. Epub 2010 May 6. 10.1016/j.cub.2010.03.055 PubMed 20451386