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pBAD33-Gm
(Plasmid #65098)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65098 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBAD33
  • Backbone manufacturer
    Beckwith Lab
  • Modifications to backbone
    the gentamicin resistance gene (aacC1) of the pUCGmlox vector was used. pUCGmlox was digested with SmaI, and the fragment containing the lox-flanked aacC1 gene was purified and cloned into the PvuII and ScaI sites in previously digested pBAD33, replacing its chloramphenicol resistance gene (cat)
  • Vector type
    Bacterial Expression
  • Promoter pBAD

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    grow in 10ug/ul Gentamycin. Too much gentamycin is toxic for e. coli.
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD33-Gm was a gift from Juan Tomas (Addgene plasmid # 65098 ; http://n2t.net/addgene:65098 ; RRID:Addgene_65098)
  • For your References section:

    Genetics and proteomics of Aeromonas salmonicida lipopolysaccharide core biosynthesis. Jimenez N, Lacasta A, Vilches S, Reyes M, Vazquez J, Aquillini E, Merino S, Regue M, Tomas JM. J Bacteriol. 2009 Apr;191(7):2228-36. doi: 10.1128/JB.01395-08. Epub 2009 Jan 16. 10.1128/JB.01395-08 PubMed 19151135